Cameraless high-throughput three-dimensional imaging flow cytometry
نویسندگان
چکیده
منابع مشابه
The Application of Imaging Flow Cytometry to High-Throughput Biodosimetry
Biodosimetry methods, including the dicentric chromosome assay, the cytokinesis-block micronucleus assay and the γH2AX marker of DNA damage are used to determine the dose of ionizing radiation. These techniques are particularly useful when physical dosimetry is absent or questioned. While these assays can be very sensitive and specific, the standard methods need to be adapted to increase sample...
متن کاملAnalysis of High-Throughput Flow Cytometry Data Using plateCore
Flow cytometry (FCM) software packages from R/Bioconductor, such as flowCore and flowViz, serve as an open platform for development of new analysis tools and methods. We created plateCore, a new package that extends the functionality in these core packages to enable automated negative control-based gating and make the processing and analysis of plate-based data sets from high-throughput FCM scr...
متن کاملTowards High Throughput Selection of Binding Ligands: Using Flow Cytometry
Phage display libraries represent a relatively easy way to generate binding ligands against a vast number of different targets. Although in principle, phage display selection should be amenable to automation, this has not yet been described and present selection protocols are far from high throughput. We have examined the selection process in a systematic approach and attempted to automate each...
متن کاملHigh-throughput flow cytometry data normalization for clinical trials.
Flow cytometry datasets from clinical trials generate very large datasets and are usually highly standardized, focusing on endpoints that are well defined apriori. Staining variability of individual makers is not uncommon and complicates manual gating, requiring the analyst to adapt gates for each sample, which is unwieldy for large datasets. It can lead to unreliable measurements, especially i...
متن کاملHigh-Throughput Flow Cytometry for Predicting Drug-Induced Hepatotoxicity
The development of a new drug is a long, expensive and complex process which aims to identify a pharmacologically-active low toxicity drug candidate. Large amount of resources and time are wasted if a drug fails in late stages of development or is withdrawn from the market because of toxicity. Hepatotoxicity in particular is a frequent cause for the failure of a drug to get approved, or for the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optica
سال: 2019
ISSN: 2334-2536
DOI: 10.1364/optica.6.001297